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XIV (D)

 FOURTH LETTER

Pavia, 20 December, 1795

 

 

SOURCES
PRINTED MANUSCRIPT
Cant. Disc. P. 71

 

Copy with Turin Sci. Ass. (B. XII, 10)

Cart. Volt. J 28, J 33, L 16.

REMARKS


TITLE:

DATE: In the MS copy with Tur. Sci. Ass. we read: Pavia 20 December 1795. In J33 (first minute) there is: Como, 30 October

 _______________________

J 28 consists in a single sheet with annotations on experiments introducing people into the Circuit.

J33 L 16. Extremely lengthy minute.

 The MS copy at the Tor. Sci Ass. has been corrected by comparing with  the Volt. File  Manuscripts J33, L16.+

COPY OF ANOTHER LETTER FROM
ALESSANDRO VOLTA
To Abbé ANTON MARIA VASSALLI
Dated, Pavia 20 December,

ON ANIMAL ELECTRICITY

I have already indicated and explained in the previous letter that the simplest and easiest way to excite convulsions and shaking in the frog’s legs, without using any metals or first class conductors, a few moments after preparing the frog in the best way, so that the leg may remain united to the trunk through the sciatic nerves, is to fold one leg or the other and put it in contact either with the above-mentioned nerves or the dorsal muscles. In any case, this is not always successful nor in all frogs, even if they are very lively and recently prepared for the experiment. Moreover, to be successful, two conditions are required, one slightly less than necessary, the other absolutely indispensable, viz. 1st, the part of the leg which has to touch those nerves or the dorsal muscles must not be fleshy substance but a part of the tendon or ligament proceeding from the gastrocnemius muscle, which, completely bared, extends to the extremity of the foot. 2nd,  blood or some other glutinous or saline humour must be put in between the contacts. Now here is the gathering of three conductors of different kinds, but all of the second class, as required in order to excite and set the electric fluid in motion. That is how the above two conditions reduce to only one: three different conductors forming a circle. In the experiment we are dealing with, these three conductors are smooth, hard tendon matter on the one hand, on the other either soft nerve or flesh with not too smooth a surface and glutinous or saline humour in between. The three are so different from one another that the electric current, though still very weak, is able sensibly to stimulate the crural nerves of a newly prepared frog, as explained, prepared in such a way that the current must pass through the narrow way of those very nerves. In the event that one of those circumstances should be missing (either that the frog is not prepared in the suggested way or that, after some minutes, its excitability has remarkably decreased, or there is no interlacing of three different conductors or, finally, they are not different enough) the convulsions do not occur.

 Leaving aside what refers to the excitability of the nerves and muscles of the frog, which must be very big for the experiments we are speaking of to be successful, as I have suggested more than once, let us consider particularly the last circumstance, namely that we need the coincidence of three different conductors [1], but they must all be very different; and let us see which ones come out to be more or less suitable in this regard.

Well then, the experiment is really successful when any part of the strong, compact tendon with a white, bright surface, originating from the gastrocnemius muscle and extending beyond the articulation of the foot like a pearl-coloured lamina, is opposed to the delicate dull white sciatic nerves and the reddish, tender flesh of the trunk. Obviously, they must not be in direct contact but there must be either blood or other glutinous or saline humour between them. The experiment, I say, is very successful, as it is often possible to excite convulsions in the frog’s legs even some minutes after it has been prepared properly. On the contrary, it gives a very bad result if we set the fleshy parts of the leg or thigh, and also the gastrocnemius muscle itself from the mid point upwards, against the afore-mentioned nerves or, worse, against any fleshy part of the trunk. Indeed the result is so bad that you hardly ever get the convulsions, even if you interpose the best of the glutinous or saline humours, the one which makes the result easier, that is a strong alkali.

I say hardly ever because it happens sometimes. Occasionally you can excite convulsions also with the contact of muscles against nerves and muscles against muscles dirty with blood or other glutinous or saline humour (as one of these must always be put between, which I have pointed out more than once). Such is the experiment recalled in previous letters and which is due to the author of the work On the Use and Activity of the Conducting Arc, etc., where the sciatic nerves, either alone or with a piece of backbone tied to them, hang down out of the thighs and you make those nerves or this piece of backbone knock against the thighs themselves, in this way exciting convulsions in all the leg muscles from top to bottom, especially if the parts have been previously moistened with salt water. Such and similar are also Dr. VALLI’s experiments in which a person, holding a frog by its feet with one damp hand or in the mouth, touches the sciatic nerves or the piece of backbone hanging therefrom with a finger of the other hand, or better with the tongue; or else the person dipping the aforementioned backbone with part of the nerves in water in a big glass completes the circle by dipping a finger in the same water or by having it dipped by another person with whom that person forms a chain holding hands, etc. But these experiments happen quite rarely and I’d almost say by a miracle. This is the situation: you will be able to stimulate muscular movements very many times in quite a lot of frogs, though not very lively ones, observing the conditions I have described, chiefly the one making the sciatic nerves or any part of the bare, bloodstained trunk touch the  tendon where the gastrocnemius muscle ends. I say you will be successful a thousand times using such a method and just once, in some frogs endowed with singular excitability,  if you act in other ways, even when making the sciatic nerves or the trunk touch the leg itself directly but not where the tendon divides. In short, you can never promise yourself success without getting a contact between some parts of this tendon and said nerves or trunk, one or other of which, or the tendon itself, must be dirty with blood or some other glutinous or saline humour. The interposing of this third conductor different from both the conductors between which it lies is the other condition required.

But finally, even when you manage to excite convulsions in those other ways the indispensable condition I have pointed out will always be required: three or more different conductors interlaced; and the great principle I have stated will always occur, that is to say that it is from the contact of different conductors that the action of setting the electric fluid in motion is born.. As a matter of fact, also when one muscle faces another, if there be some even merely superficial difference between them in consistency, in the humour they are bathed or simply smeared with, or else if you find between them not one but two different humours which join in that place, then either of such accidental differences may suffice to give an impetus to the electric fluid or to determine very weak electric current. This extremely weak current may go so far as to excite convulsions in on of those extremely sensitive frogs. Here is how also those very rare cases can be explained and how none of the experiments of this kind can be objected to me as opposing the principle I have stated and demonstrated by other more numerous experiments, in which you need more evident differences between adjoining conductors  in the circuit so that convulsions may occur – differences more remarkable than those which may perchance occur between one muscle and another or between a muscle and a nerve, etc.

Let us now pass on to deal with the most indispensable condition in greater detail, viz that there must not be direct contact of the leg, that is of the tendon coming from the gastrocnemius muscle, with the sciatic nerves or with the fleshy parts of the trunk, but there must be a third conductor interposed, different in kind, but it also belonging to the second class and also being very different, which we pointed out as being necessary in order to be successful. so this intermediate conductor has to be  neither pure water nor a humour little different from water, nor any substance soaked in such  a humour. These watery conductors are very little different from this or that animal conductor, I mean from the outer surface of the tendon or nerve which fit together, that is from the mostly watery humour moistening these animal parts and making them conductors ( since, when dry, they no longer conduct). They differ so much as to hardly move and extremely weak current that, by chance, in once case out of a thousand, could only just move an extremely weak electric current capable of stimulating convulsions in some newly prepared frogs endowed with prodigious sensitivity; but in the other 999 experiments, it will not be able to produce the slightest muscular movement. To give rather stronger motion to the electric flow and to induce an efficacious current to shake even frogs endowed with normal excitability, provided they are perfectly and recently prepared, it is necessary that the intermediate conductor between the leg or its major tendon and the sciatic nerves or the dorsal muscles be either sticky blood or other humour notably different from water.

That is why the experiment succeeds not seldom in a frog dirtied with blood seeping from cuts after dissection and even better when this blood, having become less watery, turns out to be more slimy and sticky; for it no longer succeeds after the frog has been prepared and cleaned by splashing and rubbing it with fresh water, as I have already written in the previous letter. The tests succeed again if we dry off the water and once more soil that part which has to be brought into contact with sticky blood or other glutinous or saline humour. The situation is this: when, for lack of blood or other humour very different from water, you obtain no convulsions in the frog  however you make one of its legs or the tendon coming from the gastrocnemic muscle touch either the sciatic nerves  or one or other part of the trunk, you are successful many times when deliberately dirtying either the said nerves or some part of the spine with  sticky, thick blood , saliva or better salty water and getting the contact of the folded leg in exactly the place which is covered with the afore-mentioned humours.  

It is easier to be successful if you soil either of those parts with soap dissolved in a little water, somewhat better still if you wet it with some drops of concentrated acid, and especially with a strong alkaline liquid, so much so that in this manner (of course the tendon is included in the contact) there is no frog – even weak – in which I cannot succeed in exciting convulsions, even many minutes after preparation.  Results from the experiments produced up to now, and from many others I will produce later on, show that  a thin layer of such humours suffices between the place where the leg, or, as indicated, its big tendon, on the one hand,  meets the sciatic nerves or any fleshy part of the trunk, on the other; only if the contact between these two parts of the animal is not direct.   In short, it is enough if the two parts be scarcely covered or veiled at the points where contact has to occur. But so little is sufficient that not even a lot can be prejudicial; I mean that it is not detrimental that either one or both parts of the animal which have to contact should be plentifully covered with that humour, meaning that a mass, as great as you will, is placed between. The condition is that one of such conductors must be interposed.  It matters not if it is thick or thin, long or short; all the effect depends on the touching surfaces . So the experiment turns out equally well, keeping the whole trunk of the frog, or part of it, immersed in a bigger or smaller bath of blood, salt water or half-dissolved soap, etc., but especially saturated alkaline solution , and bending the leg so that some parts of the big tendon coming from the gastrocnemius muscle (this condition is very important and must always be respected) come to touch the liquid itself at any place. 

Here are some more, nearly equivalent ways. Lay the trunk of your frog down on a piece of soap whose surface has been moistened so that it forms a semi-liquid pulp. Fold one of its legs and press it on this pulp at any distance from the trunk, taking care, as usual, that the contact be in the places near the foot joint or in some other part of the well-known tendon. In this manner you will surely succeed, as I do, in  exciting convulsions in nearly all freshly prepared frogs, several times in succession, by alternating between touching and removing contact.   Success is obtained for longer in the following manner, which I often put into practice.      

I properly soak a piece of sponge or a strip of cardboard, cloth, leather or, better, tinder (amadou) in a strong alkaline solution and lay one part of it onto the sciatic nerves or any part of the trunk, applying it very well.  The more or less long rest of this soaked strip stands out from the trunk so that we can make the leg touch it easily along some part of the usual tendon. So every time I  make this contact I excite more or less vigorous contractions and spasms in all the muscles of the leg; and this happens also in remarkably weakened frogs and those prepared some time before. In very lively and recently cut frogs, convulsions are so violent, the shaking and tossing so strong, that they are surprising. It is possible to keep on getting them for a long time, when a) you would not get them  not only by means of a simple metallic conductor used instead of an alkaline solution, or even two pieces of the same metal,  not even by means of two slightly different metals like silver and gold, brass and iron, lead and tin, when you apply one of them to the dorsal muscles and the other one to the thigh muscle.     

  I must not fail to warn that the convulsions one tries to excite by the afore-mentioned contacts sometimes do not occur during the first moments after applying either the alkaline liquid, the softened soap or the salt water, etc., to one or other part of the frog in the way described. The convulsions occur after some time, when the humour applied has penetrated or at least has come into more perfect contact with that part of the animal which it covers and soaks. If this part is too soaked and dripping with watery humour  when the alkaline liquid or the salty water is applied thereto, the experiments turn out worse and results come later due to the fact that the watery humour dilutes the other salty or soapy liquid too much at the place where the two layers merge together; the salty or soapy liquid is no longer the same, no longer is such quantity as it should be at that place, unlike the animal conductors between which it is interposed , or unlike the humour that makes the animal substances into conductors. On the contrary, if the quantity of salty or soapy liquid is far more than the quantity of water wetting that part of the leg or trunk of the frog, the result not obtained at first comes some time later, i.e. when that little water is distributed throughout the great quantity of the other liquid or soap, so that it somehow disappears therein, till that wet conductor which has been interposed proves to be different enough from the animal parts it is in contact with.

 I will also draw your attention to the fact that when very salty water is used, and more so with acid and alkaline liquids, the muscles of the frog which are touched and infiltrated by them remarkably feel the effects of such stimuli, so that some tremors, throbbing and jerking are to be observed. Yet such convulsions are limited to those muscles and fibres which the liquid irritates locally and do not pass to the parts far away (from the trunk, I mean, even if it is soaked in it), to the hind legs. These convulsions can be only too well distinguished from the others which are bigger and more extensive and shake all the leg muscles, from top to bottom, and make them leap any time one of them is bent so that its main tendon touches the liquid or the soaking parts of the trunk. By such fitting together we set the electric current flowing.

On the other hand, we can get the acrid, stimulating liquid not to penetrate or prick b) any muscle of the animal, and even less its nerves, thus avoiding any irritation and offence that the liquid may cause. It is enough to put in touch with the trunk of the frog a piece of fresh, juicy flesh from any animal, a piece of curd, hard-boiled egg white, polenta, a strip of cardboard, skin or tinder soaked in water, a bit of juicy fruit, etc., in short any second class conductor (but it must be a good conductor) and onto some points of said conductor applied to the trunk of the frog put the salty liquid we are talking about on a bit of sponge or tinder well soaked in it. After disposing things this way, bring the leg of the frog or the usual tendon into contact with the liquid or the sponge soaked in it. The convulsions will occur nearly in the same way as if the liquid itself were in direct contact with the frog’s trunk. I say nearly in the same way, supposing that the body applied to the trunk be both a good conductor, as I have already mentioned, and not very long, as I add now, since otherwise, slowing down the electric current, it causes its action to be less efficient.

The reverse of this last experiment is to apply the conductor we wish to add, whatever it may be, i.e. a piece of flesh, curd, polenta, etc., no longer to the frog’s trunk but to one of its legs, particularly to the tendon I have mentioned so many times, and to put on some parts of this interposed body the alkaline liquid or a sponge drenched in it. Then put any part of the bare trunk in contact with this liquid.

Not very different from that is the experiment already mentioned, when you hold the frog in your damp hand by its feet and you touch the dorsal muscles or other part of the trunk with one finger of your second hand, wet with blood, salt water or dissolved soap or better with a strong alkaline liquid.. As I have already pointed out, it is vary rarely, and only in some especially excitable frogs, that we may be successful in arousing convulsions, using this method. This happens for both the reasons already mentioned, viz. because the electric current is considerably slowed down, if not held up, by the poor conductivity of the skin on our hands (even though moistened on the surface),  as well as the long distance the current has to travel from one hand to the other through the person’s body. If several people form the circuit, namely two, three, four, hand in hand making a chain, the success of the experiment will be much more difficult, in proportion as the impediments increase. Nevertheless, the convulsions also occur in this way, when the frog is endowed with the highest excitability, as I have said.

Moreover, experiments using two, three or more people [and] different metals again show that the skin of the hands is a remarkable impediment to the flowing of the current, as it is not sufficiently soaked in humours and therefore does not conduct electricity well. These experiments (which you also repeated more than one year ago and which I have been showing for three years) do not obviously produce the effect, if the interlocked hands  are not streaming with perspiration or, failing that, are not moistened properly. But since the cutis, though bathed thus on the surface, remains deficient in humours within, that is in the layers under the first moistened one, until you reach the live, juicy flesh, there is still some resistance to the electric current, obstructing it and making it a little slower; so that if this current is already very weak, as in the experiments we are now concerned with, when metal conductors (I mean of the first class) are not used, and also in those experiments two or more metal ones are used but not much different from one another, it is not astonishing that the current be made completely ineffective to arouse convulsions in frogs still endowed, if not with most remarkable and rare, with more than mediocre excitability.

In case you should wish to introduce one of the most acrid and irritating salty liquids between the conductors where the circuit closes, and at the same time to avoid any part of the frog from being penetrated – and not even lightly, fleetingly touched by the liquid itself – you can apply a piece of the already mentioned substances, that is flesh cut from another animal, curdled milk, or polenta, etc. to the frog’s legs (mainly over the big tendon) and another piece to the trunk .  Then bring these two pieces, or added conductors, into contact so that the circuit closes and there is between them a thick or thin layer of the liquid. Also in this way – susceptible of many variations, as can be understood – muscle contractions and shaking of the legs occur in a lot of propitious circumstances.

I have purposed to describe, most renowned Colleague,  not all but the main ways I have varied the tests to stimulate convulsions in the frog, thanks to fitting together only conductors of the second class. Now one of these ways I have not dealt with yet, either in this or the previous letter, a way which, on the other hand, agrees with the one I have described above, is to dip one or two legs of the recently, properly prepared frog into a beaker full of water, while the trunk, or part thereof, is immersed in another beaker, and then to induce communication between water and water by means of other conductors of the same second class but different from water.

Just to give an idea of many experiments performed by me in this manner, it is sufficient to say, in general, that when a conducting arc was made of just one piece or of several pieces all of the same material (e.g. all made of one, two or more pieces of the same fresh, juicy flesh, or the same nerve or tendon, or the whole made of pieces of hard-boiled egg-white, polenta, cheese, etc.) or one or more people with hands linked together, dipping a clean finger or one moistened with simple water into each of the two beakers, convulsions never took place. On very few occasions, convulsions were excited when one of the fingers or one end of the other homogeneous conducting arc (mainly the end which touched the water in which the trunk of the frog was immersed) had been soiled with sticky blood or another glutinous or saline humour, half-dissolved soap, or better a thick alkaline liquid. I say on very few occasions, that is only in the most propitious circumstances, with frogs gifted with extraordinary excitability and during the first attempts with the same most recently properly prepared with alkali, have I been able to be successful, even a short while after and in not particularly sensitive frogs.

That is sufficient about such experiments in which, as in all others, putting together simply different conductors is necessary. Only two conductors, however different, forming all the circuit are not enough, as the actions against each other balance out, in two similar matching ones. As can be seen in Figure 1 attached hereto, in which two bodies aa,bb fit together, whatever the action on the electric fluid may be (I mean the current that originates from such fitting together), having to be alike from the two opposite parts, either the current tends to pass from a to b or from b to a, from both parts, no current can flow from right to left or from left to right, owing to the fact that such opposite forces counter-balance.

Nothing happens either when the arc bb, applied to the wet conductor aa, is metallic but made of the very same metal, with its ends bb,  alike not only in substance but also in temper, cleanness, etc., which I dealt with at length in the first letter last year.

Well then, we need three or more different bodies entering the conductor circuit in order to determine the flow of current, since the action which pushes the electric fluid from left to right or from right to left prevails against the other which pushes in the opposite direction, in virtue of the different matching. In other words, it is necessary that the conductor aa be placed between two, bb and cc , different from it and from each other, as in Figure 2 – and the more different the better.

If there are more than three different conductors put together, or any number, (Figs. 3 and 4) electric current will be produced which is more or less active, that is to say capable or incapable of arousing convulsions, etc. according to and in proportion as the forces tending to move the electric fluid in one direction prevail over the ones tending in the opposite direction. The flow will be completely absent only in very rare cases, when the sum of forces on one side properly balance the sum of the opposite forces.

But let’s leave this subject for the moment. We will return to it at greater length another time. It is useful to point out here that if one of the interconnecting conductors is of higher conductivity, e.g. a strong saline solution, and especially a pure alkali, which is so far the most active of the second class conductors (as zinc is among the first class ones), and if this conductor lies between two conductors of very different kinds from it and also remarkably different from one another, nearly all the experiment depends on the fitting together of this conductor with these latter ones, so that we can say the matching together of however many other conductors, slightly dissimilar from each other and not very active, counts for nothing or very little. Such is the case of the frog partially immersed in two beakers of water. Water as a conductor, or rather, according to its conductivity,  is so little different from the green, juicy body of the frog that they can both be considered as one single watery conductor. If you dip a slice of juicy meat, hard-boiled egg-white, curd, polenta, etc. into the two beakers (as in the experiment described above), the action on the electric fluid that we get by such juxtapositioning, I mean by the contact of these bodies in the water, is not something we can rely on a lot. Those bodies/contacts/conductivities decide that they generate where an either thick or thin layer of dissolved soap, strong acid or alkaline liquid applied to the other end of such conducting arcs, that is to say that the layer covers and wraps the other extremity of the slice of flesh, polenta, etc., is brought into contact with the water in the other beaker; the fact is that the soap or alkaline liquid is kept and pressed between the water (here) and the conductor different from water, towards each of which it acts very differently. In short, all or nearly all the game is played by those three most remarkably dissimilar bodies. So, in Figure 4, if aa is the body of water in the beaker, cc a prepared frog, bb a slice of juicy flesh, pp a piece of cheese or hard-boiled egg, zz a drop or a thin or thick layer of alkaline liquid, the effect will almost entirely depend on the network of three bodies, aa, zz, pp, that is on the very different actions which arise at the dissimilar connection points, I say, at the connecting faces za and zp. Little or nothing will happen at the other connections of faces ab, bc, cp, as none of the conductors aa, bb, cc, is very different in its conductivity c)

Anyway, a little difference is enough to cause some movement in the electric fluid and to produce whatever current therein, as has already been said. This current is always weak when the circuit is made up of second class conductors, however different they may be in kind, as I have pointed out several times. It is extremely weak in all cases when no conductor in the chain is very different from the two between which it lies. Therefore, if there is no glutinous or alkaline conductor (which are precisely the conductors that differ most in quality from the others of the second class) never or hardly ever can we stimulate convulsions even in the liveliest and most recently prepared of frogs. I say hardly ever because it may happen that some frogs, most freshly prepared and excitable to the highest degree, move even by means of that weak electric current   which is aroused at the joining points of those conductors which are little different from one another.                                

To sum up, any time it happens that we stimulate convulsions in a frog’s legs, connected to the trunk only by the crural nerves, by creating another communication between the legs and the trunk through a conducting arc made of as many pieces as you wish, you will always find that there are some remarkable differences between the two, or better the three such contiguous conductors which the circuit is made of. This includes the parts of the frog’s trunk themselves, which are also more or less different from one another as to the property we are dealing with, individually the sinewy part compared with the muscular and the nerves, the acqueous and weak humours compared with the sticky and salty humours, as I have pointed out from the beginning. Now when I can demonstrate – I’ll say more – when it can be merely assumed that three or more conductors, though little different from one another, enter the circuit, this is enough to confirm the statement, which I make and maintain, that motion is given to the electric fluid by juxtaposing different conductors and not by any charge or imbalance in the animal organs or between the inner side and the outer side thereof, as GALVANI has thought and his followers still claim. To demonstrate the theory of this professed animal electricity, which I declare does not exist and which I think I have demolished effectively and completely by means of many experiments, replacing it by my other principle of artificial electricity roused by an external cause, my opponents ought to show me that convulsions occur in frogs when the circuit is formed by conductors all of the same kind, and in nothing differing from one another – which they will never be able to do. On the contrary, I can always show, as I have already said, that convulsions occur when there is the conjunction of three or more conductors of different kinds.

I drafted this letter more than a month ago, as a continuation of the other, but so far I have had no time to transcribe it. I’ll stop here for the time being, but others will follow.

________________________________

NOTES BY THE BOARD OF EDITORS

AND ADDITIONS FROM VOLTA’S MANUSCRIPTS

  __________

 

[1] At about this point in Ms. J33 we find the following digression:

[… let’s talk a little about three conductors and why two aren’t different enough?]

It’s easy to understand if you think that when only two conductors form a circuit, like A and B in the appended figure, the actions that occur at the points of contact of A itself with B itself, at the two opposite ends, destroy each other, being alike. Therefore no electric current can flow from right to left or from left to right.  

So, even if A is a metal, provided that it is alike at its two ends, not only in kind but also in temper, polish, etc., and B the body of the frog, provided that the parts on which said ends of arc A press are similar, no motion occurs in the frog itself, however excitable it is; since the contact of a metal with a wet conductor, though strongly stimulating the electric fluid, does so from both parts in opposite directions and with equal strength, and therefore cannot make it flow.

Remark: the first sentence, within square brackets,  can be read in the Ms., although it has been rubbed out.

Translation by M_Luise Guerrini 

Revised and completed by John Coggan



a) Translator’s note: Volta here means “whereas”.

b) Translator’s note: sic

c) Translator’s note: literally “its motor virtue”

 

   

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